Journal: International Journal of Breast Cancer

Article Title: Comparison of Fluorescence In Situ Hybridization and Chromogenic In Situ Hybridization for Low and High Throughput HER2 Genetic Testing

doi: 10.1155/2013/368731

Figure Lengend Snippet: Different characteristics between the five HER2 genetic assays.

Article Snippet: The HER2 genetic testing was performed using 5 assays: HER2 CISH pharmDx Kit-SK109, HER2 FISH pharmDx Kit-K5331, HER2 IQ-FISH pharmDx-K5731 (Dako, Denmark), ZytoDot 2C SPEC HER2 /CEN17 Probe Kit-C-3022-40 and SPEC HER2 /CEN17 Dual Colour Probe Kit-Z-2020 (ZytoVision, Germany).

Techniques: Blocking Assay, Fluorescence, Hybridization

Journal: International Journal of Breast Cancer

Article Title: Comparison of Fluorescence In Situ Hybridization and Chromogenic In Situ Hybridization for Low and High Throughput HER2 Genetic Testing

doi: 10.1155/2013/368731

Figure Lengend Snippet: Performance of mean CISH and mean FISH HER2 ratio assay.

Article Snippet: The HER2 genetic testing was performed using 5 assays: HER2 CISH pharmDx Kit-SK109, HER2 FISH pharmDx Kit-K5331, HER2 IQ-FISH pharmDx-K5731 (Dako, Denmark), ZytoDot 2C SPEC HER2 /CEN17 Probe Kit-C-3022-40 and SPEC HER2 /CEN17 Dual Colour Probe Kit-Z-2020 (ZytoVision, Germany).

Techniques: Amplification

Journal: International Journal of Breast Cancer

Article Title: Comparison of Fluorescence In Situ Hybridization and Chromogenic In Situ Hybridization for Low and High Throughput HER2 Genetic Testing

doi: 10.1155/2013/368731

Figure Lengend Snippet: Discrepancy cases between the five different HER2 genetic ratio assays.

Article Snippet: The HER2 genetic testing was performed using 5 assays: HER2 CISH pharmDx Kit-SK109, HER2 FISH pharmDx Kit-K5331, HER2 IQ-FISH pharmDx-K5731 (Dako, Denmark), ZytoDot 2C SPEC HER2 /CEN17 Probe Kit-C-3022-40 and SPEC HER2 /CEN17 Dual Colour Probe Kit-Z-2020 (ZytoVision, Germany).

Techniques:

Journal: American Journal of Clinical Pathology

Article Title: HER2 Testing and Clinical Decision Making in Gastroesophageal Adenocarcinoma

doi: 10.1093/ajcp/aqw206

Figure Lengend Snippet: Guideline Statements and Strength of Recommendation

Article Snippet: The Dako pharmaDx HER2 FISH kit was used for the ToGA trial, 3 and there is considerable experience with FISH in testing for HER2 amplification in breast carcinomas.

Techniques: Blocking Assay, Biomarker Assay, Expressing, Activity Assay

Journal: American Journal of Clinical Pathology

Article Title: HER2 Testing and Clinical Decision Making in Gastroesophageal Adenocarcinoma

doi: 10.1093/ajcp/aqw206

Figure Lengend Snippet: Algorithm for pathologists. Tumor cell cluster is defined as a cluster of five or more tumor cells. Additional recommendations: Pathologists should ensure that biopsy or resection specimens used for HER2 testing are rapidly placed in fixative, ideally within 1 hour (cold ischemic time), and are fixed in 10% neutral buffered formalin for 6 to 72 hours. Routine histology processing and HER2 testing should be performed according to analytically validated protocols. Pathologists should identify areas of invasive adenocarcinoma and also mark areas with strongest intensity of HER2 expression by immunohistochemistry (IHC) in the gastroesophageal adenocarcinoma (GEA) specimen for subsequent scoring when in situ hybridization (ISH) is required.

Article Snippet: The Dako pharmaDx HER2 FISH kit was used for the ToGA trial, 3 and there is considerable experience with FISH in testing for HER2 amplification in breast carcinomas.

Techniques: Expressing, Immunohistochemistry, In Situ Hybridization

Journal: American Journal of Clinical Pathology

Article Title: HER2 Testing and Clinical Decision Making in Gastroesophageal Adenocarcinoma

doi: 10.1093/ajcp/aqw206

Figure Lengend Snippet: Scoring Guidelines for Interpretation of HER2 Immunohistochemistry in Gastric Carcinoma a

Article Snippet: The Dako pharmaDx HER2 FISH kit was used for the ToGA trial, 3 and there is considerable experience with FISH in testing for HER2 amplification in breast carcinomas.

Techniques: Immunohistochemistry, Expressing, Staining

Journal: American Journal of Clinical Pathology

Article Title: HER2 Testing and Clinical Decision Making in Gastroesophageal Adenocarcinoma

doi: 10.1093/ajcp/aqw206

Figure Lengend Snippet: HER2 immunochemistry showing representative cases for scoring. A, Negative 0: no reactivity, specifically no membranous reactivity is seen in any of the tumor cells. Any cytoplasmic staining is disregarded for scoring purposes. B, Negative 1+: tumor cells with faint/barely perceptible membranous staining. C, Equivocal 2+: tumor cells with weak to moderate, complete, basolateral, and lateral membranous staining. Columnar cells that are sectioned tangentially tend to show a complete membranous staining pattern. D, Positive 3+: tumor cells with a strong, complete, basolateral, and lateral membranous reactivity. Also note that cells showing a complete membranous staining pattern are often tangentially sectioned columnar cells (HER2, ×40 [A, C, and D] and ×20 [B]).

Article Snippet: The Dako pharmaDx HER2 FISH kit was used for the ToGA trial, 3 and there is considerable experience with FISH in testing for HER2 amplification in breast carcinomas.

Techniques: Staining

Journal: American Journal of Clinical Pathology

Article Title: HER2 Testing and Clinical Decision Making in Gastroesophageal Adenocarcinoma

doi: 10.1093/ajcp/aqw206

Figure Lengend Snippet: HER2 and CEP17 FISH show scores of representative cases. A, Not amplified: ratio 1.0. Mean number of HER2 signals per cell is 1.9; mean number of CEP17 signals per cell is 1.8. B, Not amplified: ratio 1.3. Mean number of HER2 signals per cell is 3.4; mean number of CEP17 signals per cell is 2.7. Segmental duplication (or polysomy) likely accounts for signal numbers greater than two per cell. C, Amplified: ratio 3.0. Mean number of HER2 signals per cell is 5.2; mean number of CEP17 signals per cell is 1.7. CEP, chromosome enumeration probe; FISH, fluorescence in situ hybridization.

Article Snippet: The Dako pharmaDx HER2 FISH kit was used for the ToGA trial, 3 and there is considerable experience with FISH in testing for HER2 amplification in breast carcinomas.

Techniques: Amplification, Fluorescence, In Situ Hybridization

Journal: American Journal of Clinical Pathology

Article Title: HER2 Testing and Clinical Decision Making in Gastroesophageal Adenocarcinoma

doi: 10.1093/ajcp/aqw206

Figure Lengend Snippet: Key Reporting Elements a

Article Snippet: The Dako pharmaDx HER2 FISH kit was used for the ToGA trial, 3 and there is considerable experience with FISH in testing for HER2 amplification in breast carcinomas.

Techniques: Immunohistochemistry, In Situ Hybridization, Amplification, Sequencing, Mutagenesis, Expressing